Bay Biosciences provides high quality, clinical grade fully characterized fresh frozen buffy coat, PBMC samples from patients diagnosed with breast cancer using customized collection and processing protocols for research, development and discovery.

Moreover, detailed clinical data, biomarker information associated with the samples is provided o a valued customer for development and research. This includes, CT scans, biopsy, serology and pathology annotations, and brest cancer patients history associated with the buffy coat samples.

What is Buffy Coat?

A buffy coat is the thin, yellowish-white layer of leukocytes (white blood cells) and platelets (thrombocytes) that forms between the red blood cells and plasma after centrifugation of anticoagulated blood. Furthermore, this concentrated layer is a valuable resource for various applications, including DNA extraction, because it contains nucleated white blood cells.

In addition, in the blood banking industry, blood banks often collect buffy coats from whole blood donations to produce platelet concentrates for transfusion, especially for patients with cancer, trauma, or hematological diseases.

Buffy Coat is processed from patients’ whole blood samples collected in EDTA anticoagulant tubes using centrifugation. After the centrifugation process, a layer of clear fluid, the plasma, comes on top; meanwhile, a layer of red fluid (Erythrocytes), which contains most of the red blood cells, stays at the bottom.

Additionally, a very thin white layer in between is the buffy coat. Each of the buffy coats is less than 1% of the total blood volume. Notably, the buffy coat is so-called because it is usually buff in hue.

  • Top Layer – Plasma: 55% of total Blood Volume
  • Middle Layer – Buffy Coat: Leukocytes and Platelets <1% of total Blood Volume
  • Bottom Layer – Erythrocytes: 45% of total Blood Volume

Furthermore, the buffy coat consists of white blood cells and platelets, and it is a concentration of leukocyte suspension. Importantly, the buffy coat is used to extract DNA from the blood of mammals, since mammalian red blood cells are anucleate and do not contain DNA.

Consequently, this offers an option for purifying large amounts of gDNA (genomic DNA) from relatively small sample sizes. Additionally, buffy coat preparation is often the first step towards further processing for T-cell depletion, tumor cell purging, or cryopreservation for both marrow and blood hematopoietic progenitor cell (HPC) collections.

Preparation

Buffy coat is derived by processing whole blood using the following procedure:

  • First, collect anticoagulated blood: A technician collects blood into tubes containing an anticoagulant.
  • Next, centrifuge the blood: The technician centrifuges the blood sample at high speed.
  • Then, separate the layers: This process separates the blood into three distinct layers:
  • Specifically, Plasma: (top layer)
  • Followed by, Buffy coat: (middle, thin layer of white blood cells and platelets)
  • And finally, Erythrocytes: or red blood cells (bottom layer)

Components of a Buffy Coat

The buffy coat contains a mix of immune cells, including:

  • Firstly, Granulocytes: Neutrophils, basophils, and eosinophils.
  • Additionally, Lymphocytes: B cells, T cells, and natural killer cells.
  • Moreover, Monocytes: White blood cells that can develop into macrophages or dendritic cells.
  • Finally, Platelets (Thrombocytes): Small cell fragments important for blood clotting.

Applications

Following are some of the applications of buff coats:

  • For instance, Research: Buffy coats are a rich source of nucleated cells for DNA extraction and other molecular studies.
  • Moreover, Medical Diagnostics: They can be used to concentrate microfilariae (parasitic worms) from blood samples for diagnosis.
  • Additionally, Therapeutics: Buffy coats are processed to create pooled platelet transfusions, which are critical for patients who cannot produce enough platelets on their own.

Biospecimens

biospecimens

Bay Biosciences is a global leader in providing researchers with high quality, clinical grade, fully characterized human tissue samples, bio-specimens, and human bio-fluid collections.

Moreover, human biospecimens are available including tumor tissue, serum, plasma and PBMC samples from most other therapeutic areas.

Furthermore, Bay Biosciences maintains and manages its own biorepository, the human tissue bank (biobank) consisting of thousands of diseased samples (specimens) and likewise normal healthy donors for controls. Additionally, available in all formats and types.

In fact, our biobank procures and stores fully consented, de-identified and institutional review boards (IRB) approved human tissue samples, human biofluids such as serum samples, plasma samples from various diseases and matched controls.

Also, all our human tissue collections, human biospecimens and human biofluids are provided with detailed, samples associated patient’s clinical data.

In fact, this critical patient’s clinical data includes information relating to their past and current disease, treatment history, lifestyle choices, biomarkers, and genetic information.

Additionally, researchers find the patient’s data associated with the human biospecimens extremely valuable and use it to help identify new effective treatments (drug discovery & development) in oncology, as well as in other therapeutic areas and diseases.

Bay Biosciences banks wide variety of human tissue samples and human biological samples, including fresh frozen human biospecimens cryogenically preserved at – 80°C.

For example fresh frozen tissue samples, tumor tissue samples, formalin-fixed paraffin-embedded (FFPE), tissue slides, with matching human bio-fluids, whole blood and blood-derived products such as human serum, human plasma and human PBMCs.

Bay Biosciences is a global leader in collecting and providing human tissue samples according to the specified requirements and customized, tailor-made collection protocols.

Please contact us anytime to discuss your special research projects and customized human tissue sample requirements.

Types of Biospecimens

Bay Biosciences provides human tissue samples (human specimens) and human biofluids from diseased and normal healthy donors which includes:

Moreover, we can also procure most human biospecimens and human biofluids, special collections and requests for human samples that are difficult to find. All our human tissue samples and human biofluids are procured through IRB-approved clinical protocols and procedures.

In addition to the standard processing protocols, Bay Biosciences can also provide human biofluids such as  human plasma, human serum, and human PBMCs bio-fluid samples using custom processing protocols; you buy donor-specific collections in higher volumes and specified sample aliquots from us.

Bay Biosciences also provides human biospecimens from normal healthy donors; volunteers, for controls and clinical research, Contact us Now.