Serum Plasma Biofluid Processing Protocol

BAY BIOSCIENCES, LLC.

       

     SERUM AND PLASMA PROCESSING PROTOCOL

Sample Collection and Processing Overview: Standard protocol for serum and plasma collection will be as follows:

1).Confirm and make sure the signed and dated Bay Biosciences LLC., informed consent form (ICF) was obtained from the patient.

2). Draw whole blood in one 10 mL serum separator collection tube (tiger top).

3). Draw whole blood in one 10 mL EDTA or K2EDTA blood collection tube (lavender top), and invert 6 to 8 times at room temperature.

Steps 4-11 must be carried out within 30-60 minutes of collection to prevent biochemical changes.

4). Centrifuge the tubes for 20 minutes at 1300 x g (approximately 3250 RPM)  to separate the serum/plasma from the cells.

Aliquoting

5). Pipette a minimum of 1 mL serum from the tiger top (serum separator) tube into each pre-labelled aliquot vial. Depending on the hematocrit of the patient, one 10 mL tube of blood yields 3 to 5 aliquots of serum in volumes of 1 mL per vials. The average yield from 10 mL blood collection is total of 4 mL of serum.

6). Record the pre-labeled cryovial donor identification number (DIN) on the procurement log and label as serum.

7). Pipette a minimum of 1mL plasma from the lavender top (EDTA) tube into each pre-labeled aliquot vial. Depending on the hematocrit of the patient, one 10 mL tube of blood yields 3 to 5 aliquots of plasma in volumes of 1 mL per vials. The average yield from 10 mL blood collection is a total of 4 mL of plasma.

8). Record the pre-labeled cryovial donor identification number (DIN) on the procurement log and label as plasma.

9). Pipette a minimum of 1 mL of the peripheral blood cells that remain in the bottom of the lavender top (EDTA) tube into a pre-labeled cryovial.

10). Record the pre-labeled cryovial donor identification number on the procurement log and label as cells.

11). Snap-freeze aliquots and store frozen at -20° C or lower. If liquid nitrogen is unavailable, place samples directly into -80° C freezer unit. It is important to avoid freeze-thaw cycles.

Biospecimens

Bay Biosciences is, indeed a global leader in providing researchers with high quality, clinical grade, fully characterized human tissue samples, bio-specimens, and human bio-fluid collections.

Specifically, aamples available include cancer (tumor) tissue, cancer serum, cancer plasma, cancer, peripheral blood mononuclear cells (PBMC) and human tissue samples from most other therapeutic areas and diseases.

Moreover, Bay Biosciences maintains and manages its own biorepository, the human tissue bank (biobank) consisting of thousands of diseased samples (specimens) and from normal healthy donors available in all formats and types.

In fact, our biobank procures and stores fully consented, de-identified and institutional review boards (IRB) approved human tissue samples and matched controls.

Additionally, all our human tissue collections, human specimens and human bio-fluids are provided with detailed, samples associated patient’s clinical data.

In particular, critical patient’s clinical data includes information relating to their past and current disease, treatment history, lifestyle choices, biomarkers, and genetic information.

Moreover, researchers find patient’s data extremely valuable and use it to help identify new effective treatments (drug discovery & development) in oncology, and other therapeutic areas and diseases.

Specifically, Bay Biosciences banks wide variety of human tissue samples and biological samples, including cryogenically preserved at – 80°C.

For example fresh frozen tissue samples, tumor tissue samples, formalin-fixed paraffin-embedded (FFPE), tissue slides, with matching human bio-fluids, whole blood and blood-derived products such as serum, plasma and PBMC.

Furthermore, Bay Biosciences is a global leader in collecting and providing human tissue samples according to the specified requirements and customized, tailor-made collection protocols.

Therefore, please contact us anytime to discuss your special research projects and customized human tissue sample requirements.

Types of Biospecimens

Bay Biosciences provides human tissue samples (human specimens) from diseased and normal healthy donors which includes:

  • Firstly, Peripheral whole-blood
  • Secondly, Amniotic fluid
  • Third, Bronchoalveolar lavage fluid (BAL)
  • Moreover, Sputum
  • Furthermore, Pleural effusion
  • Next, Cerebrospinal fluid (CSF)
  • Also, Serum (sera)
  • Likewise, Plasma
  • In addition, Peripheral blood mononuclear cells (PBMC)
  • For example, Saliva
  • Also, Buffy coat
  • Moreover, Urine
  • Furthermore, stool samples
  • Next, Aqueous humor
  • Likewise, Vitreous humor
  • Lastly, Kidney stones (renal calculi)
  • Finally, Other bodily fluids from most diseases including cancer.

Moreover, we can also procure most human bio-specimens, furthermore; we offer special collections and requests for human samples that are difficult to find. All our human tissue samples are procured through IRB-approved clinical protocols and procedures.

In addition to the standard processing protocols, Bay Biosciences can also provide human plasma, serum, and PBMC bio-fluid samples using custom processing protocols. Additionally you buy donor-specific collections in higher volumes and specified sample aliquots from us.

Furthermore, Bay Biosciences also provides human samples from normal healthy donors; volunteers, for controls and clinical research, contact us Now.

 

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