BAY BIOSCIENCES, LLC.
BAY BIOSCIENCES, LLC. 
SERUM AND PLASMA PROCESSING PROTOCOL
Sample Collection and Processing Overview: Standard protocol for serum and plasma collection will be as follows:
1).Confirm and make sure the signed and dated Bay Biosciences LLC., informed consent form (ICF) was obtained from the patient.
2). Draw whole blood in one 10 mL serum separator collection tube (tiger top).
3). Draw whole blood in one 10 mL EDTA or K2EDTA blood collection tube (lavender top), and invert 6 to 8 times at room temperature.
Steps 4-11 must be carried out within 30-60 minutes of collection to prevent biochemical changes.
4). Centrifuge the tubes for 20 minutes at 1300 x g (approximately 3250 RPM) to separate the serum/plasma from the cells.
5). Pipette a minimum of 1 mL serum from the tiger top (serum separator) tube into each pre-labelled aliquot vial. Depending on the hematocrit of the patient, one 10 mL tube of blood yields 3 to 5 aliquots of serum in volumes of 1 mL per vials. The average yield from 10 mL blood collection is total of 4 mL of serum.
6). Record the pre-labeled cryovial donor identification number (DIN) on the procurement log and label as serum.
7). Pipette a minimum of 1mL plasma from the lavender top (EDTA) tube into each pre-labeled aliquot vial. Depending on the hematocrit of the patient, one 10 mL tube of blood yields 3 to 5 aliquots of plasma in volumes of 1 mL per vials. The average yield from 10 mL blood collection is a total of 4 mL of plasma.
8). Record the pre-labeled cryovial donor identification number (DIN) on the procurement log and label as plasma.
9). Pipette a minimum of 1 mL of the peripheral blood cells that remain in the bottom of the lavender top (EDTA) tube into a pre-labeled cryovial.
10). Record the pre-labeled cryovial donor identification number on the procurement log and label as cells.
11). Snap-freeze aliquots and store frozen at -20° C or lower. If liquid nitrogen is unavailable, place samples directly into -80° C freezer unit. It is important to avoid freeze-thaw cycles.
Bay Biosciences is a global leader in providing researchers with high quality, clinical grade, fully characterized human tissue samples, bio-specimens and human bio-fluid collections from cancer (tumor) tissue, cancer serum, cancer plasma cancer PBMC and human tissue samples from most other therapeutic areas and diseases.
Bay Biosciences maintains and manages it’s own bio-repository, human tissue bank (biobank) consisting of thousands of diseased samples (specimens) and from normal healthy donors available in all formats and types. Our biobank procures and stores fully consented, deidentified and institutional review boards (IRB) approved human tissue samples and matched controls.
All our human human tissue collections, human specimens and human bio-fluids are provided with detailed samples associated patient’s clinical data. This critical patient’s clinical data includes information relating to their past and current disease, treatment history, lifestyle choices, biomarkers and genetic information. Patient’s data is extremely valuable for researchers and is used to help identify new effective treatments (drug discovery & development) in oncology, other therapeutic areas and diseases. This clinical information is critical to demonstrate their impact, monitor the safety of medicines, testing & diagnostics, and generate new knowledge about the causes of disease and illness.
Bay Biosciences banks wide variety of human tissue samples and biological samples including cryogenically preserved -80°C, fresh, fresh frozen tissue samples, tumor tissue samples, FFPE’s, tissue slides, with matching human bio-fluids, whole blood and blood derived products such as serum, plasma and PBMC’s.
Bay Biosciences is a global leader in collecting and providing human tissue samples according to the researchers specified requirements and customized, tailor made collection protocols. Please contact us anytime to discuss your special research projects and customized human tissue sample requirements.
Bay Biosciences provides human tissue samples (human specimens) from diseased and normal healthy donors; including peripheral whole-blood, amniotic fluid, bronchoalveolar lavage fluid (BAL), sputum, pleural effusion, cerebrospinal fluid (CSF), serum (sera), plasma, peripheral blood mononuclear cells (PBMC’s), saliva, Buffy coat, urine, stool samples, aqueous humor, vitreous humor, kidney stones, renal calculi, nephrolithiasis, urolithiasis and other bodily fluids from most diseases including cancer. We can also procure most human bio-specimens and can do special collections and requests of human samples that are difficult to find. All our human tissue samples are procured through IRB approved clinical protocols and procedures.
In addition to the standard processing protocols Bay Biosciences can also provide human plasma, serum, PBMC bio-fluid samples using custom processing protocols, you can buy donor specific sample collections in higher volumes and specified sample aliquoting from us. Bay Biosciences also provides human samples from normal healthy donors, volunteers, for controls and clinical research, contact us Now.
日本のお客様は、ベイバイオサイエンスジャパンBay Biosciences Japanまたはhttp://baybiosciences-jp.com/contact/までご連絡ください。