Amniotic Fluid Samples for Research
Amniotic Fluid Overview
Amniotic fluid is a clear, yellow fluid which is found within the first 12 days following conception within the amniotic sac. It surrounds the growing baby in the uterus.
Amniotic fluid also contains vital components, such as nutrients, hormones, and infection-fighting antibodies.
When amniotic fluid is green or brown, this indicates that the baby has passed meconium before birth. Meconium is the name of the first bowel movement.
Meconium in the fluid can be problematic. It can cause a breathing problem called meconium aspiration syndrome that occurs when the meconium enters the lungs. In some cases, babies will require treatment after they are born.
Functions of Amniotic Fluid
- Infection control: The amniotic fluid contains antibodies
- Lubrication Amniotic fluid prevents parts of the body such as the fingers and toes from growing together; webbing can occur if amniotic fluid levels are low.
- Lung and digestive system development: By breathing and swallowing the amniotic fluid, the baby practices using the muscles of these systems as they grow.
- Muscle and bone development: As the baby floats inside the amniotic sac, it has the freedom to move about, giving muscles and bones the opportunity to develop properly.
- Protecting the fetus: The fluid cushions the baby from outside pressures, acting as a shock absorber.
- Temperature control: The fluid insulates the baby, keeping it warm and maintaining a regular temperature.
- Umbilical cord support: Fluid in the uterus prevents the umbilical cord from being compressed. This cord transports food and oxygen from the placenta to the growing fetus.
When the waters break, the amniotic sac tears. The amniotic fluid contained within the sac then begins to leak out via the cervix and vagina.
Amniotic Fluid Disorders
Some conditions can cause there to be more or less than the normal amounts of amniotic fluid.
Oligohydramnios: This is a condition when there is too little amniotic fluid.
Polyhydramnios: This condition is also referred to as hydramnios or amniotic fluid disorder, is when there is too much fluid.
Oligohydramnios
- Birth defects, such as kidney abnormalities
- Chronic high blood pressure (Hypertension)
- Delivering past the due date
- Diabetes
- Prior growth-restricted pregnancies
- Lupus
- Multiple pregnancies, for example twins or triplets
- Other unknown reasons, known as idiopathic
- Problems with the placenta, for example, abruption
- Preeclampsia
Oligohydramnios can happen during any trimester but is a more concerning problem during the first 6 months of pregnancy. During that time, there is a higher risk of birth defects, loss of pregnancy, preterm birth, or neonatal loss of life.
If fluid levels are low in the last trimester, the risks include:
- Labor complications
- Slow fetal growth
- The need for a Cesarean delivery
Doctors may use the following tests:
- Nonstress tests: This is to check the baby’s heartbeat when it is resting and when it is moving.
- Biophysical profiling: An ultrasound scan can detect the baby’s movements, muscle tone, breathing and amniotic fluid levels. This may be followed up with a nonstress test.
- Fetal kick counts: This is to time how long it takes for the baby to kick a certain number of times.
- Doppler studies: These use sound waves to check the flow of blood in the baby.
In some cases, doctors may decide that labor will need to be induced, in order to protect the mother or the child. Amnioinfusion (the infusion of saline into the uterus), increasing maternal fluids, and bed rest may also be necessary.
There is a higher chance of labor complications, due to the risk of umbilical cord compression. Amnioinfusion may be needed during labor. In some cases, a cesarian delivery may be necessary.
Polyhydramnios
- Achondroplasia, a bone growth disorder
- Brain or nervous system disorders, such as anencephaly or myotonic dystrophy
- Beckwith-Wiedemann syndrome, which is a congenital growth disorder
- Fetal heart rate problems
- Gastrointestinal disorders, including duodenal or esophageal atresia, gastroschisis, and diaphragmatic hernia
- Infection
- Fetal lung abnormalities
- Hydrops fetalis, in which an abnormal level of water builds up inside multiple body areas of a fetus
- Twin-to-twin transfusion syndrome, where one child gets more blood flow than the other
- Mismatched blood between mother and child, for example Rh incompatibility or Kell diseases
Poorly controlled maternal diabetes also increases the risk.
Other complications include the following:
- Cord prolapses
- Fetal malposition
- Preterm labor
- Premature rupture of membranes
- Placental abruption
- Stillbirth
- Postpartum hemorrhage
Testing for maternal diabetes may be recommended, and frequent ultrasounds will be obtained to monitor the levels of amniotic fluid in the uterus.
Mild cases of polyhydramnios typically resolve without treatment.
Leaking Amniotic Fluid
Sometimes, what looks like fluid leaking is actually urine, because the uterus is pressing on the bladder.
If the fluid has no color and no smell, it will be amniotic fluid, and you should contact a healthcare provider as labor will normally begin soon.
If the fluid is green, brownish-green, or foul-smelling, this may indicate the presence of meconium or an infection.
Premature Rupture of Membranes
This is known as premature rupture preterm, but it is also possible to have premature rupture at term. That is when 37 weeks or more of pregnancy are complete, but labor does not start spontaneously within 6 hours of the membrane rupturing.
It is important to seek medical help as soon as possible and to avoid having sex or introducing anything into the vagina, as this could lead to an infection.
Anyone who is concerned about leaking or levels of amniotic fluid during pregnancy should discuss this with their doctor.
- Peripheral whole-blood
- Amniotic fluid
- Bronchoalveolar lavage fluid (BAL)
- Sputum
- Pleural effusion
- Cerebrospinal fluid (CSF)
- Serum (sera)
- Plasma
- Peripheral blood mononuclear cells (PBMC)
- Saliva
- Buffy coat
- Urine
- Stool samples
- Aqueous humor
- Vitreous humor
- Kidney stones (renal calculi)
- Other bodily fluids from most diseases including cancer.
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